BETTERPLUS EDUCATION

  PREPARATION OF BLOOD FILMS ON SLIDES Blood films should be made on clean glass slides. Films made on coverglasses have negligible advantages and are unsuitable for modern laboratory practice. Films may be spread by hand or by means of an automated slide spreader, the latter being either a stand-alone instrument or a component of an automated blood cell counter. Manual method Blood films can be prepared from fresh blood with no anticoagulant added or from ethylenediaminetetra-acetic acid (EDTA)-anticoagulated blood. Heparinised blood should not generally be used because its staining charac- teristics differ from those of EDTA-anticoagulated blood. Good films can be made in the following manner using clean slides, if necessary wiped free from dust immedi- ately before use. Slides should measure 75 × 25mm and be approximately 1mm thick; ideally, they should be frosted at one end to facilitate labelling, but these are more expensive. First, make a spreader from a glass slide that has...

  BLOOD COLLECTION BY CAPILLARY PUNCTURE A Blood test is prescribed in most of the blood-related disorders, metabolic disorders and in various infections as well as for the Regular health Check-ups. Whenever a small amount of blood is required for the tests, instead of venipuncture, a capillary puncture is done to collect the sample. In Children and Adults, finger puncture is done to collect the blood sample whereas, in Infants, Heel puncture is done to obtain the specimen. I) BLOOD COLLECTION BY FINGER PUNCTURE REQUIREMENTS FOR THE FINGER PUNCTURE Blood Lancet Alcohol/Spirit Swab Surgical Gauze Microscope Glass Slide Capillary tube Glass Marking pencil All the above-mentioned things should be ready in hand before starting the procedure. PROCEDURE OF THE FINGER PUNCTURE ⇒ Firstly ensure that the patient should be in a comfortable position. ⇒ Find a spot on the Middle or Ring finger of the left hand (as Most peoples are Right-handed). ⇒ Clean the selected site for the puncture with ...